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KMID : 0368419880310020091
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Journal of Plant Biology
1988 Volume.31 No. 2 p.91 ~ p.100
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Effect of Ca^Ⅱ+ and Polyamines on the Activity of β-Glucan Synthetase Ⅱ Related to Cell Wall Synthesis in Carrot Suspension Cultured Cells
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Abstract
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The effect of Ca^2+ and polyamines on the activity of β-glucan synthetase Ⅱ(GSⅡ) related to cell wall synthesis was studied in carrot suspension cultured cells.
The activity of GS Ⅱ is four times higher than that of β-glucan synthetase Ⅰ in carrot suspension cultured cells and in vitro experiment, the activity of GSⅡ was increased in response to increase in concentration of Ca^2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca^2+ and polyamines. When carrot suspension cultured cells were incubated together with Ca^2+ and polyamines, the GSⅡ activity was high at 0.1mM of Ca^2+ and 1 mM of putrescine.
Also, polycationic poly-L-lysine and poly-L-ornithine increased about 50% the GSⅡ activity than that of the control, respectively. These results may imply that Ca^2+ and polyamines were related to the enzyme activity as a polycationic nature. In addition, verapamil as the calcium channel blocker and flunarizine as an antagonist of calcium mechanism in cytoplasm decreased GSⅡ activity ramarkably, Ca^2+ and calmodulin stimulated GSⅡ activity as Ca^2+ of free ion rather than Ca^2+ calmodulin complex.
The effect of 2,4-D on the GSⅡ activity in culture medum is shown to be low at 0.1㎎ per liter and GSⅡ activity increased about 30% more than that of the 0.1㎎/l at the range of 0.3-1.0㎎ per litere.
Cummulative results suggest that Ca^2+ and polyfamines stimulate the cell wall synthesis by means of the enhancement of GSⅡ activity responsible for synthesizing the cell wall components.
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